While on non birding matters, Martin and I were doing our bottom BBS square, on Saturday, we came across this suspected Peziza growing in a wood chip path, on Aberdare Golf Club land.
I took a cup home and as well as obtaining a spore print, I did a Melzer's reagent test on the asci (the spore bearing tubes). I cut thin sections of a small pied of the cup and mounted them on a slide, in distilled water, placing a cover slip on top. After examining the sections like that, I took it back off the microscope and with a tissue, drew some Melzer's reagent under the slip, so that it replaced the water. After leaving it a minute or so, I drew more distilled water through, using the same process, this time the water replacing the Melzer's.
When put te slide back under the microscope and examined the asci, I could see that the very tips of them were stained blue.
|These two images were taken at a magnification of 200x (each division of the|
scale equals 5 microns) The blue tips of the asci can just be made out. It was
more obvious when looking through the microscope.
The blue reaction confirmed that the fungus is a Peziza, but as to which of the sixty-odd species, that remains to be seen.